SPF鸡感染禽白血病病毒A/B亚群后血清抗体与卵黄抗体的变化及其相关性

doi:10.11843/j.issn.0366-6964.2014.04.016

Abstract

Abstract:

The aim of this study was to study the feasibility of yolk antibody indicating the ALV infection status in SPF chickens instead of serum antibody.Eggs and serum samples were collected one to one correspondence from 40 23-weeks-old SPF chickens，which 21 chickens were positive to ALV-Ab and 19 chickens were negative.The egg yolk was diluted into 1∶150，1∶200，1∶300 respectively to compare the correlation of yolk antibody and serum antibody in the ELISA tests.76 SPF chickens with different ALV-Ab level were breed separately，eggs were collected once a week and serum was collected every three weeks from each chicken during the age of 25 to 34 weeks，totally 836 egg yolks and 304 serum samples were collected.They were tested the antibody response to ALV-Ab by ELISA detection kits.The yolk antibody correlated well with serum antibody when the egg yolk was diluted at the 1:300 dilution otherwise the false negative or false positives may arise.Antibody response to ALV-Ab in 836 egg yolks correlated well with 304 serum samples during the age of 25 to 34 weeks and the consistency rate is 94.7%.The yolk antibody can be as a good index to estimate the ALV infection in SPF chickens instead of serum antibody.

CLC Number:

S852.659.3

ZHAO Peng， LI De-qing， DONG Xuan， XU Hai-peng， CUI Zhi-zhong. Dynamic of Yolk Antibody and Serum Antibody and Their Correlation in SPF Chickens Infected with Avian Leukosis Virus Subgroup A/B[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(4): 614-620.

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Dynamic of Yolk Antibody and Serum Antibody and Their Correlation in SPF Chickens Infected with Avian Leukosis Virus Subgroup A/B

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